Supplementary Material for Thudi et al. (2011)

Reference:

Thudi M, Bohra A, Nayak SN, Varghese N, Shah TM, Penmetsa RV, Nepolean T, Gudipati S, Gaur PM, Kulwal PL, Upadhyaya HD, Kavi Kishor PB, Winter P, Kahl G, Town CD, Kilian A, Cook DR and Varshney RK (2011) Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1291 marker loci for chickpea (Cicer arietinum L.).

Genetic map details in CMap

1: Detailed picture of the comprehensive inter-specific map of chickpea (ICC 4958 × PI 489777). The map comprised 1291 loci with an average inter-maker distance of 0.65cM spanning a total distance of 845.56cM. Each linkage group is divided into 10cM bins which help to select highly informative SSRs in a systematic way, that represents the whole genome as well as enhance the probability of displaying higher polymorphism in the germplasm to be analysed.


2: Comparison of linkage group 1 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Seventeen marker loci on LG 1 of Winter et al. 2000 and seven marker loci of LG 1 of Millan et al. 2010 are common to the LG 1 of Thudi et al. 2011.


3: Comparison of linkage group 2 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Seventeen marker loci on LG 2 of Winter et al. 2000 and 17 marker loci of LG 2 and one marker locus of LG 1 of iMillan et al. 2010 are common to the LG 2 of Thudi et al. 2011.


4: Comparison of linkage group 3 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Nineteen marker loci on LG 3 of Winter et al. 2000 and 13 marker loci of LG 3 of Millan et al. 2010 are common to the LG 3 of Thudi et al. 2011.


5: Comparison of linkage group 4 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Sixteen marker loci on LG 4, one marker locus of LG 11 of Winter et al. 2000 and seven marker loci of LG 4b of Millan et al. 2010 are common to the LG 4 of Thudi et al. 2011


6: Comparison of linkage group 5 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Seventeen marker loci on LG 7 of Winter et al. 2000 and 10 marker loci of LG 5 of Millan et al. 2010 are common to the LG 5 of Thudi et al. 2011.


7: Comparison of linkage group 6 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Seven marker loci on LG 6 of Winter et al. 2000 and three marker loci of LG 6 of Millan et al. 2010 are common to the LG 6 of Thudi et al. 2011.


8: Comparison of linkage group 7 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Nine marker loci on LG 5, two marker loci on LG 15 of Winter et al. 2000 and one marker locus on LG 6 of Millan et al. 2010 are common to the LG 7 of Thudi et al. 2011.


9: Comparison of linkage group 8 of the comprehensive map with that of Winter et al. (2000) and Millan et al. (2010). Seven marker loci on LG 8 of Winter et al. 2000 are common to the LG 8 of Thudi et al. 2011 and none of the linkage groups of Millan et al. 2010 possessed correspondence to Thudi et al. 2011.

Supplementary files submitted with MS

Table S1: Details on novel simple sequence repeat (SSR) markers developed after mining bacterial artificial chromosome (BAC)- end sequences

Table S2: Features of 5397 polymorphic DArT loci based on marker analysis in 94 genotypes

Table S3: Number of markers on each linkage group that show segregation distortion

Table S4: Bin wise description of high density inter-specific map (ICC 4958 × PI 489777) of chickpea

Table S5: List of 112 chickpea genotypes used for analysis of SSR (1-48) and DArT markers (19-112)